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Listar por autor "García-Suárez, María del Mar"
Mostrando ítems 1-11 de 11
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Detection of Streptococcus pneumoniae in Urine by Loop-Mediated Isothermal Amplification
Cima-Cabal, María Dolores ; Vázquez-Espinosa, Emma; Vázquez, Fernando; García-Suárez, María del Mar (Journal of Pediatric Infectious Diseases, 01/2021)Objective To assess the loop-mediated isothermal amplification (LAMP) to detect cell-free DNA from Streptococcus pneumoniae in urine samples from children with pneumococcal pneumonia. Methods LAMP reactions using four ... -
Developing Diagnostic and Therapeutic Approaches to Bacterial Infections for a New Era: Implications of Globalization
Fernández, Lucía; Cima-Cabal, María Dolores ; Catarina Duarte, Ana; Rodríguez, Ana; García, Pilar; García-Suárez, María del Mar (Antibiotics-Basel, 12/2020)In just a few months, the current coronavirus pandemic has exposed the need for a more global approach to human health. Indeed, the quick spread of infectious diseases and their unpredictable consequences, in terms of human ... -
Electrocatalytic Palladium Nanoclusters as Versatile Indicators of Bioassays: Rapid Electroanalytical Detection of SARS-CoV-2 by Reverse Transcription Loop-Mediated Isothermal Amplification
Rodríguez-Penedo, Alejandro; Rioboo-Legaspi, Pablo; González-López, Andrea; Lores-Padín, Ana; Pereiro, Rosario; García-Suárez, María del Mar; Cima-Cabal, María Dolores; Costa-Rama, Estefania; Fernández, Beatriz; Fernández-Abedul, M. Teresa (Advanced Healthcare Materials, 2023)Quantitative polymerase chain reaction (qPCR) is considered the gold standard for pathogen detection. However, improvement is still required, especially regarding the possibilities of decentralization. Apart from other ... -
Electrochemical Detection for Isothermal Loop-Mediated Amplification of Pneumolysin Gene of Streptococcus pneumoniae Based on the Oxidation of Phenol Red Indicator
Gonzalez-Lopez, Andrea; Cima-Cabal, María Dolores; Rioboo-Legaspi, Pablo; Costa-Rama, Estefania; García-Suárez, María del Mar; Fernandez-Abedul, M. Teresa (Analytical Chemistry, 2022)A highly sensitive electrochemical methodology for end-point detection of loop-mediated isothermal nucleic acid amplification reactions was developed. It is based on the oxidation process of phenol red (PR), commonly used ... -
Gram-positive pneumonia: Possibilities offered by phage therapy
Fernández, Lucía; Cima-Cabal, María Dolores ; Catarina Duarte, Ana; Rodríguez, Ana; García-Suárez, María del Mar ; García, Pilar (MDPI, 2021)Pneumonia is an acute pulmonary infection whose high hospitalization and mortality rates can, on occasion, bring healthcare systems to the brink of collapse. Both viral and bacterial pneumonia are uncovering many gaps in ... -
Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism
García-Suárez, María del Mar; González-Rodríguez, Irene; Cima-Cabal, María Dolores; Yuste, Jose Enrique; Vázquez, Fernando; Santiago, Enrique (Diagnostics, 18/11/2019)Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without ... -
Magnetic Nanoclusters Increase the Sensitivity of Lateral Flow Immunoassays for Protein Detection: Application to Pneumolysin as a Biomarker for Streptococcus pneumoniae
Salvador, Maria; Marques-Fernandez, Jose Luis; Bunge, Alexander; Martinez-Garcia, Jose Carlos; Turcu, Rodica; Peddis, Davide; García-Suárez, María del Mar ; Cima-Cabal, María Dolores ; Rivas, Montserrat (Nanomaterials, 06/2022)Lateral flow immunoassays for detecting biomarkers in body fluids are simple, quick, inexpensive point-of-care tests widely used in disease surveillance, such as during the coronavirus disease 2019 (COVID-19) pandemic. ... -
Phenol red as electrochemical indicator for highly sensitive quantification of SARS-CoV-2 by loop-mediated isothermal amplification detection
Rioboo-Legaspi, Pablo; González-López, A.; Beltrán-Sánchez, J.F.; Cima-Cabal, María Dolores; García-Suárez, María del Mar; García Sánchez, A.J.; Fernández-Otero, T.; García Haro, J.; Costa-Rama, Estefania; Fernández-Abedul, M. Teresa (Talanta, 2024)The current COVID-19 pandemic has made patent the need for rapid and cost-effective diagnostic tests, crucial for future infectious outbreaks. Loop-mediated isothermal amplification (LAMP) is a promising and decentralized ... -
Pneumolysin as a target for new therapies against pneumococcal infections: A systematic review
Cima-Cabal, María Dolores; Molina, Felipe; López-Sánchez, José Ignacio; Pérez-Santín, Efrén; García-Suárez, María del Mar (PLoS ONE, 2023)Background This systematic review evaluates pneumolysin (PLY) as a target for new treatments against pneumococcal infections. Pneumolysin is one of the main virulence factors produced by all types of pneumococci. This toxin ... -
Protein Expression Analysis by Western Blot and Protein–Protein Interactions
Cima-Cabal, María Dolores ; Vázquez, Fernando; de los Toyos, Juan R; García-Suárez, María del Mar (Methods in Molecular Biology, 2019)Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and ... -
Toxicity prediction based on artificial intelligence: A multidisciplinary overview
Peréz-Santín, Efren ; Rodríguez Solana, Raquel; González García, Mariano ; García-Suárez, María del Mar ; Blanco Díaz, Gerardo David ; Cima-Cabal, María Dolores ; Moreno Rojas, José Manuel; López-Sánchez, José Ignacio (John Wiley and Sons Inc, 2021)The use and production of chemical compounds are subjected to strong legislative pressure. Chemical toxicity and adverse effects derived from exposure to chemicals are key regulatory aspects for a multitude of industries, ...