Protein Expression Analysis by Western Blot and Protein–Protein Interactions
Autor:
Cima-Cabal, María Dolores (1)
; Vázquez, Fernando
; de los Toyos, Juan R
; García-Suárez, María del Mar (1)
Fecha:
2019Palabra clave:
Tipo de Ítem:
bookPartResumen:
Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections. Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
Descripción:
Capítulo del libro "Iovino F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY"
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