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    Protein Expression Analysis by Western Blot and Protein–Protein Interactions

    Autor: 
    Cima-Cabal, María Dolores (1)
    ;
    Vázquez, Fernando
    ;
    de los Toyos, Juan R
    ;
    García-Suárez, María del Mar (1)
    Fecha: 
    2019
    Palabra clave: 
    monoclonal antibody; pneumolysin; PpmA; urine; western-blot; Scopus(2)
    Tipo de Ítem: 
    bookPart
    URI: 
    https://reunir.unir.net/handle/123456789/10558
    DOI: 
    https://doi.org/10.1007/978-1-4939-9199-0_9
    Dirección web: 
    https://link.springer.com/protocol/10.1007%2F978-1-4939-9199-0_9
    Resumen:
    Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections. Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
    Descripción: 
    Capítulo del libro "Iovino F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY"
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