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Protein Expression Analysis by Western Blot and Protein–Protein Interactions

Autor: 
Cima-Cabal, María Dolores
;
Vázquez, Fernando
;
de los Toyos, Juan R
;
García-Suárez, María del Mar
Fecha: 
2019
Palabra clave: 
Revista / editorial: 
Methods in Molecular Biology
Citación: 
Cima-Cabal M.D., Vazquez F., de los Toyos J.R., del Mar García-Suárez M. (2019) Protein Expression Analysis by Western Blot and Protein–Protein Interactions. In: Iovino F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9199-0_9
Tipo de Ítem: 
bookPart
URI: 
https://reunir.unir.net/handle/123456789/10558
DOI: 
https://doi.org/10.1007/978-1-4939-9199-0_9
Dirección web: 
https://link.springer.com/protocol/10.1007%2F978-1-4939-9199-0_9
Resumen:
Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections. Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
Descripción: 
Capítulo del libro "Iovino F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY"
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