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Listar por autor "Vázquez, Fernando"
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Detection of Streptococcus pneumoniae in Urine by Loop-Mediated Isothermal Amplification
Cima-Cabal, María Dolores ; Vázquez-Espinosa, Emma; Vázquez, Fernando; García-Suárez, María del Mar (Journal of Pediatric Infectious Diseases, 01/2021)Objective To assess the loop-mediated isothermal amplification (LAMP) to detect cell-free DNA from Streptococcus pneumoniae in urine samples from children with pneumococcal pneumonia. Methods LAMP reactions using four ... -
Identification of Pneumococcal Serotypes by PCR–Restriction Fragment Length Polymorphism
García-Suárez, María del Mar; González-Rodríguez, Irene; Cima-Cabal, María Dolores; Yuste, Jose Enrique; Vázquez, Fernando; Santiago, Enrique (Diagnostics, 18/11/2019)Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without ... -
Protein Expression Analysis by Western Blot and Protein–Protein Interactions
Cima-Cabal, María Dolores ; Vázquez, Fernando; de los Toyos, Juan R; García-Suárez, María del Mar (Methods in Molecular Biology, 2019)Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and ...